Universal Enzyme-Linked Immunosorbent Assays (ELISA) and Utility in the Detection of Antibodies against Salmonella spp. in Several Animal Species

The aim of this study was to confirm the feasibility using hybrid immunoglobulin-binding reagents in ELISAs for IgG/IgY detection and specific antibodies against an infectious microorganism (Salmonella spp.) various animal species a universal diagnostic ELISA. Hybrid bacterial proteins (IBP), including recombinant protein LA, LG, AG, have been produced improve their binding affinity much larger number immunoglobulins. Thus, represents powerful tool binding, detection, purification immunoglobulins fragments. However, SpLA-LG-peroxidase SpLAG-anti-IgY-peroxidase were periodate method. These compounds shown be effective as reagents. Their surpasses that previously reported IgG-binding proteins, most known SpAG, SpLA, SpLG. IgY fraction isolated from egg yolks birds, chicken, bantam hen, guinea quail, goose, duck, wild domestic pigeons, parakeets, cattle egrets, pheasants, ostrich. chloroform-polyethylene glycol (PEG) An ELISA anti-Salmonella spp. employed with some modifications determine presence humans, laying hens, geese, quails, pigeons. Salmonella is motile, flagellated, rod-shaped zoonotic pathogen can survive or absence oxygen. They belong family Enterobacteriaceae are implicated typhoid fever food-borne illnesses. This associated several diseases, which may become fatal negatively impact health individuals economies globally. poultry industry vulnerable influence pernicious microbe. authors concluded enzyme-linked immunosorbent assays reproducible detecting both avian mammalian species, but SpLAG-anti-IgY-HRP conjugate only reacted whole panel antibodies. further used standardize determination antibodies, human serologically assessed.